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icecold 1x pbs  (Thermo Fisher)


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    Structured Review

    Thermo Fisher icecold 1x pbs
    Icecold 1x Pbs, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/icecold+1x+pbs/PBS/pm41238532-313-13-23
    Average 99 stars, based on 1 article reviews
    icecold 1x pbs - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Fat:

    Article Title: Ketone body supplementation into a standard high-carbohydrate diet reduces fecundity, delays larval development, and may induce autophagy in Drosophila melanogaster.
    Article Snippet: Fasting (typically intermittent; IF), caloric restriction (CR), and the increasingly-popular high-fat, low-carbohydrate ketogenic diet (KD) count among many starvation-mimicking dietary regimens known to exert diverse effects on cellular and whole-organism behavioral, developmental, and physiological parameters.. These effects include lowering neuronal excitability, inducing the cellular-component-recycling process of autophagy, altering reproductive outcomes (especially in pathological cases), and extending lifespan.. These challenging diet regimens can produce elevated levels of circulating ketone bodies (KBs), which themselves are known to exert numerous potentially-beneficial genetic and signaling effects.

    Immunoprecipitation:

    Article Title: Oct4 is a gatekeeper of epithelial identity by regulating cytoskeletal organization in skin keratinocytes.
    Article Snippet: 1 x 106 HaCaT cells were seeded for 24 h. Then, cells were transfected with 5 mg of plasmid pcDNA3-EGFP (addgene, 13031), or pcDNA3.3-OCT4 (addgene, 26816), or pcDNA3-OCT4EGFP, or pCEP4-OCT4-WT (addgene, 40629), or pCEP4-OCT4-T234AS235A (addgene, 40630) or pCEP4-OCT4-T234E-S235E (addgene, 40631) for 48 h using FuGENE 6 (Promega, E2691) according to the manufacturer’s protocol. .. For immunoprecipitation, 6 x106 cells were seeded for 48 h. Untreated or transfected HaCaT cells were washed twice on ice with icecold 1X PBS, lysed in RIPA buffer (NaCl 150 mM, EDTA pH = 8; 5 Mm, Tris-HCl pH = 8; 50 mM, 1% Triton X-100, 0.5% sodium deoxycholate, 0.1%SDS) supplementedwith protease inhibitor tablets (ThermoFisher, A32955) for 15min on ice and centrifuged at 4 C for 15 min at 12.500 rpm. ..

    Transfection:

    Article Title: Oct4 is a gatekeeper of epithelial identity by regulating cytoskeletal organization in skin keratinocytes.
    Article Snippet: 1 x 106 HaCaT cells were seeded for 24 h. Then, cells were transfected with 5 mg of plasmid pcDNA3-EGFP (addgene, 13031), or pcDNA3.3-OCT4 (addgene, 26816), or pcDNA3-OCT4EGFP, or pCEP4-OCT4-WT (addgene, 40629), or pCEP4-OCT4-T234AS235A (addgene, 40630) or pCEP4-OCT4-T234E-S235E (addgene, 40631) for 48 h using FuGENE 6 (Promega, E2691) according to the manufacturer’s protocol. .. For immunoprecipitation, 6 x106 cells were seeded for 48 h. Untreated or transfected HaCaT cells were washed twice on ice with icecold 1X PBS, lysed in RIPA buffer (NaCl 150 mM, EDTA pH = 8; 5 Mm, Tris-HCl pH = 8; 50 mM, 1% Triton X-100, 0.5% sodium deoxycholate, 0.1%SDS) supplementedwith protease inhibitor tablets (ThermoFisher, A32955) for 15min on ice and centrifuged at 4 C for 15 min at 12.500 rpm. ..

    Protease Inhibitor:

    Article Title: Oct4 is a gatekeeper of epithelial identity by regulating cytoskeletal organization in skin keratinocytes.
    Article Snippet: 1 x 106 HaCaT cells were seeded for 24 h. Then, cells were transfected with 5 mg of plasmid pcDNA3-EGFP (addgene, 13031), or pcDNA3.3-OCT4 (addgene, 26816), or pcDNA3-OCT4EGFP, or pCEP4-OCT4-WT (addgene, 40629), or pCEP4-OCT4-T234AS235A (addgene, 40630) or pCEP4-OCT4-T234E-S235E (addgene, 40631) for 48 h using FuGENE 6 (Promega, E2691) according to the manufacturer’s protocol. .. For immunoprecipitation, 6 x106 cells were seeded for 48 h. Untreated or transfected HaCaT cells were washed twice on ice with icecold 1X PBS, lysed in RIPA buffer (NaCl 150 mM, EDTA pH = 8; 5 Mm, Tris-HCl pH = 8; 50 mM, 1% Triton X-100, 0.5% sodium deoxycholate, 0.1%SDS) supplementedwith protease inhibitor tablets (ThermoFisher, A32955) for 15min on ice and centrifuged at 4 C for 15 min at 12.500 rpm. ..

    Article Title: Inactivation of the Hippo tumor suppressor pathway promotes melanoma.
    Article Snippet: .. Cells were gently washed twice in icecold 1X PBS and lysed using ice-cold cell lysis buffer (50 mM Tris-HCl, 2% w/v SDS, 10% glycerol) containing 1X HALT (dual-phosphatase and protease inhibitor, Thermo Fisher). .. Lysates were sonicated at 20% amplitude for 20 s, diluted in 4X Sample Buffer (Boston BioProducts), and resolved using SDS gel electrophoresis.

    Article Title: In-vitro approaches to investigate the detrimental effect of light on dopaminergic neurons.
    Article Snippet: .. On day 4 cells were washed with icecold 1x PBS and lysed in M-PER (ThermoFisher Scientific) containing protease inhibitor cocktail (Roche) on ice. ..

    Lysis:

    Article Title: Inactivation of the Hippo tumor suppressor pathway promotes melanoma.
    Article Snippet: .. Cells were gently washed twice in icecold 1X PBS and lysed using ice-cold cell lysis buffer (50 mM Tris-HCl, 2% w/v SDS, 10% glycerol) containing 1X HALT (dual-phosphatase and protease inhibitor, Thermo Fisher). .. Lysates were sonicated at 20% amplitude for 20 s, diluted in 4X Sample Buffer (Boston BioProducts), and resolved using SDS gel electrophoresis.



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